Beilstein J. Nanotechnol.2017,8, 1283–1296, doi:10.3762/bjnano.8.130
depletion (STED) microscopy. Our approach allows for a step-by-step formation of dye-doped silica nanoparticles in the form of dye-incorporated spheres, which can be used as versatile fluorescent probes in confocal and STED imaging.
Keywords: bioimaging; confocal microscopy; multistepsynthesisapproach
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Scheme 1:
Pre-synthesis modification steps of the Atto647N dye. Path A: The dye was modified with a cysteic a...